About Leukocyte Alkaline Phosphatase

Endocrine Relationships of Leukocyte Alkaline Phosphatase.

Leukocyte alkaline phosphatase: electrophoretic variants associated with chronic myelogenous leukemia.

Starch-gel electrophoresis of leukocyte alkaline phosphatases, rendered soluble by treating normal leukocytes with butanol, revealed three electrophoretic variants of the enzyme. The phosphatases in similarly prepared extracts of leukemia cells differed from the normal isozymes in electrophoretic. mobility. A single variant was detected in one case of untreated leukemia; a similar component and...

Histochemical and biochemical studies on leukocyte alkaline phosphatase activity.

Fluoride and Alkaline Phosphatase

Since serum alkaline phosphatase increases in fluoride therapy for osteoporosis, it is generally accepted that fluoride stimulates bone formation. However, histochemical studies have shown that alkaline phosphatase is also increased in resorbing osteocytes. Fluoride is toxic to metabolically active bone cells, alkaline phosphatase is released, and serum alkaline phosphatase increases. We propos...

Chicken Intestinal Alkaline Phosphatase

Purified chicken intestinal alkaline phosphatase is active at pH 8 to 9, but becomes rapidly inactivated with change of pH to 6 or less. Also, a solution of the inactivated enzyme at pH 4.5 rapidly regains its activity at pH 8. In the range of pH 6 to 8 a solution of purified alkaline phosphatase consists of a mixture of active and inactive enzyme in equilibrium with each other. The rate of ina...